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IJCRR - 8(11), June, 2016

Pages: 05-09

Date of Publication: 11-Jun-2016


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GENE XPERT BASED ONE YEAR ANALYSIS OF PULMONARY KOCH'S FROM NORTH MAHARASHTRA REGION

Author: Mrudula Dravid, Sukhada Buwa, Shubhangi Dange, Hitesh Adchitre

Category: Healthcare

Abstract:Introduction: Tuberculosis is a major public health problem in the world, especially in the developing countries like India. Also MDR-TB and HIV: TB co-infection are major hurdles to achieve the aim and objectives of our national tuberculosis programme.
Objectives:
1. To diagnose Mycobacterium tuberculosis (MTB) infection in clinically suspected cases of pulmonary tuberculosis using Gene Xpert.
2. To find out HIV: TB co-infection rate.
3. To find out prevalence of Rifampicin sensitive and resistant cases in diagnosed tuberculosis patients.
4. To study factors responsible for Rifampicin resistance (MDR-TB)
Material and Methods: This retrospective study included 278 sputum samples from Jan 2015-Dec 2015 from registered RNTCP patients. The samples were subjected to Xpert MTB/RIF Assay for use with the Cepheid Gene Xpert. The Ziehl - Neelsen smear finding was provided by the RNTCP \?DOTS regional centres.
Results: A total number of 278 sputum samples were subjected to Gene Xpert analysis in the year 2015. MTB could be detected in 137 (49.28%) cases. In the rest 141 cases where MTB was not detected 14 samples reported error on Gene Xpert. Out of 278 cases, 209 (75.18%) were HIV negative, and 69 (24.82%) were HIV positive. In 69 clinically suspected HIV: TB co-infection cases, MTB could be detected in 22 (31.88%) cases. Out of these 22 cases, 4 (18.18%) were smear positive. Out of 137 MTB detected samples, 117 (85.4%) were rifampicin sensitive, 2 (1.46%) were rifampicin indeterminate resistant and 18 (13.14%) were rifampicin resistant. According to RNTCP programme criteria for suspected MDR \?TB, out of 18 MDR-TB cases, 12 (66.67%) cases were - smear positive at diagnosis , retreatment case; 3 (16.67%) cases were - any follow up smear positive, 2 (11.11%) - had contact of known MDR -TB case and 1(5.56%) - was HIV: TB case.
Conclusion: Gene Xpert was a useful tool in detection of HIV-TB co-infection. In our study out of 69 clinically suspects HIV: TB co-infection cases, one third cases were confirmed by Gene Xpert. Thus, 44 patients were not put on unnecessary AKT and were kept on follow-up. Even though the association of MDR-TB and HIV co-infection was not very significant in this study, it would not be too long before witnessing a rapid increase of MDR-TB among HIV patients if adequate and immediate measures are not taken. In the present study, MDR-TB detection rate was high among re-treatment cases. Emphasis has to be given for completion of primary treatment on time and taking proper nutrition. Patients usually stop treatment once they feel better within 2 month of starting the treatment. Special counselling and education is needed at this juncture. In our study, two cases of primary MDR-TB were from household contact. Hence, health workers must generate awareness and educate patients and family members about the risk of acquiring Primary MDR-TB to prevent its spread.

Keywords: Gene Xpert, Pulmonary tuberculosis, MDR-TB, HIV: TB co-infection

Full Text:

INTRODUCTION
India has the world’s maximum burden of TB (approximately 3.4 million cases), which accounts for one fifth of global incident cases, and ranks first among the 22 TB high-burden countries.1 Drug-resistant TB (DR-TB) is commonly seen in India; although its existence has been recognized since anti-tuberculosis drugs were first introduced for the cure of TB, adequate information regarding the prevalence of drug-resistant tuberculosis (TB) has not been reported from India.2 According to WHO Reports on global TB control, Multidrug-resistant tuberculosis (MDR-TB) in India has been reported between 2.5–2.8% amongst new TB cases and 14–17% in retreated patients.3-7 MDR-TB is a growing worldwide threat, most cases arising from a combination of physician error and patient non-compliance during treatment of susceptible TB. The amount of burden of MDR-TB varies significantly from nation to nation and region to region.8 The threat of development of active tuberculosis is high in HIV infected patients. Although antiretroviral therapy for HIV reduces this risk, TB still remains 5 times more common in HIV/AIDS persons.9 The present study was undertaken with following primary objectives: 1. To diagnose Mycobacterium tuberculosis infection in clinically suspected cases of tuberculosis using Gene Xpert 2. To find out HIV: TB co-infection rate. 3. To find out prevalence of Rifampicin sensitive and resistant cases in diagnosed tuberculosis patients. 4. To study factors responsible for Rifampicin resistance(MDR-TB)

MATERIALS AND METHODS
Study Design: Retrospective study Study period: From Jan 2015 to Dec 2015 The study was approved by Institutional Ethical Committee of SBHGMC, Dhule. Clinical data was obtained from the RNTCP filled requisition form sent along with the samples. Collection of specimen: Sputum samples from registered RNTCP patients were subjected to Xpert MTB/RIF Assay for use with the Cepheid Gene Xpert system, a semi-quantitative, nested real-time PCR in-vitro diagnostic test. After proper instruction to the patient, two sputum samples were collected per patient. The Ziehl - Neelsen smear finding was provided by the RNTCP-DOTS regional centres. The specimen were stored and transported at 2 to 8°C prior to processing whenever needed. In the laboratory, specimens were examined for the presence of obvious food particles or other solid particulates and were rejected. Processing of the samples:10 Each Xpert MTB/RIF cartridge was labelled with the sample ID. The lid of the sputum collection container was opened carefully and approximately 2 times the volume of the sample reagent (SR) to the sputum (2:1 dilution; SR: sputum) was poured in the container. The lid was replaced and shaked vigorously 10 to 20 times. The samples was incubated for 10 minutes at room temperature, and then shaked vigorously 10 to 20 times. The sample was incubated at room temperature for an additional 5 minutes. The test was started within 4 hours of adding the sample to the cartridge. The cartridge lid was opened and using the provided transfer pipette, the liquefied sample was aspirated to the line on the pipette. Care was taken so that no air bubble enters the pipette. The sample was transferred into the sample chamber of the Xpert MTB/ RIF cartridge and was dispensed slowly to minimize the risk of aerosol formation. The cartridge lid was closed firmly and loaded as per the instruction given by the manufacturer.

RESULTS
A total number of 278 sputum samples were subjected to Gene Xpert analysis in the year 2015. MTB could be detected in 137 (49.28%) cases, while in 141(50.72%) cases MTB could not be detected. In the rest 141 cases where MTB was not detected 14 samples reported error on Gene Xpert. Out of 278 cases, 209 (75.18%) were HIV negative, and 69 (24.82%) were HIV positive. In 69 clinically suspected HIV: TB co-infection cases, MTB could be detected only in 22 (31.88%). Out of these 22 HIV: TB cases, only 4 (18.18%) were smear positive (Table 1). Out of 137 MTB detected samples, 117 (85.4%) were rifampicin sensitive, 2 (1.46%) were rifampicin indeterminate resistant and 18 (13.14%) were rifampicin resistant (Figure 1). According to RNTCP programme criteria for suspected MDR –TB, out of 18 MDR-TB cases, 12 (66.67%) cases were - smear positive at diagnosis , retreatment case; 3 (16.67%) cases were - any follow up smear positive, 2 (11.11%) - had contact of known MDR -TB case and 1(5.56%) - was HIV: TB case. Out of the 22 HIV: TB co-infection cases, MDRTB was detected only in 1 case.

DISCUSSION
The WHO South-East Asia Region (SEAR), having almost one fourth of the world’s inhabitants, accounts for 38% morbidity and 39% mortality of the global burden of tuberculosis, with an approximate 4.5 million prevalent and 3.4 million incident cases and 440,000 deaths in 2013. India having a population of about 1252 million is the largest country in the Region, and is ranked first among the high-burden countries having contributed approximately 24% of the global incident TB cases and about 20% of global TB-related deaths in 2013. In 2013, the prevalence and incidence rates of all forms of tuberculosis were 211 and 171 respectively per 100,000 population.11 Thus, India is the highest TB burden nation accounting for one fifth of the worldwide incidence.12 In the present study, a total number of 278 sputum samples were subjected to Gene Xpert analysis in the year 2015. All these samples were from RNTCP programme of Dhule district. MTB could be detected in 137 (49.28%) cases, while MTB was not detected in 141 (50.72%) cases. While HIV/AIDS and tuberculosis can independently be the foremost causes for apprehension as stand-alone public health threats, the blend of the two has proven to have a far greater impact on the epidemiologic progression and subsequently on the impact it has on the global health prospect. The twin infection has been termed “accursed duet”.13 Research shows that of the opportunistic infections distressing HIV-infected patients, TB is found to be the most frequent with soaring risk for mortality.14,15 Worldwide, about 14.8% of patients with TB are co-infected with HIV.16 There is extensive inconsistency in HIV seropositivity amongst TB patients in India, ranging from 9.4% in New Delhi to 30% in Mumbai.17 Out of 278 cases, 209 (75.18%) were HIV negative, and 69 (24.82%) were HIV positive. Gene Xpert was very helpful in diagnosis of HIV: TB coinfection cases as HIV positive TB cases are more likely to be smear negative. In 69 clinically suspected HIV: TB co-infection cases, MTB could be detected in 22 (31.88%) cases. Out of these 22 cases, only 4 (18.18%) were smear positive. Sethi et al18 has reported the prevalence of HIV-TB as 20.1%, while Ghiya et al 19and Noeske et al 20 have reported the prevalence of HIV:TB as 49.2% and 32% respectively. MDR–TB is a most important challenge for TB control globally and is also linked with increased mortality and development of XDR-TB. Surveillance studies for the estimation of resistance rates and detection of MDR-TB are thus vital so as to optimize empiric drug treatment and to avoid the spreading of resistant strains in the community.21 Out of 137 MTB detected samples, 117 were rifampicin sensitive, 2 were rifampicin indeterminate resistant and 18 were rifampicin resistant i.e. rifampicin was sensitive in 85.4% of our diagnosed cases of tuberculosis and was resistant in 13.14% cases. Worldwide, 5% of TB cases were estimated to have had MDR-TB in 2014.22 While rates of MDR-TB infections are comparatively low in North America and Western Europe; they are progressively new grave dilemma worldwide, especially in areas of the Russian Federation, the former Soviet Union and other parts of Asia.23 In 2008, after China (100,000 cases), India had second highest total number of estimated MDR TB cases (99,000).24 According to WHO Reports on global TB control, MDR-TB in India continues to be reported between 2.5–2.8% and 14– 17% amongst new TB and retreated patients respectively.3-7 According to RNTCP programme criteria for suspected MDR-TB, out of 18 MDR-TB cases, 12 (66.67%) cases were - smear positive at diagnosis , retreatment case; 3 (16.67%) cases were - any follow up smear positive, 2 (11.11%) - had contact of known MDR -TB case and 1(5.56%) - was HIV: TB case. HIV may not constantly be a risk factor of MDR-TB, depending on the people investigated. In the present study, out of 22 HIV: TB confection patients only 1 case was MDR-TB. The hastening and magnifying sway of HIV infection and postponement in identification and diagnosis of tuberculosis were found to add to the outbreaks of MDR-TB among HIV infected patients in USA25 and European countries.26 However, a Thailand study 27 showed that the percentage of MDR-TB among HIV seropositives was the same as in the HIV negative group. Among the high risk groups of MDR-TB, failure of retreatment regimen and contact of MDR-TB may be the highest prospect of MDR-TB and warrant special attention. Rapid testing of resistance to rifampicin might be essentially helpful in the confirmation of MDR-TB.28

CONCLUSION
Gene expert was a useful tool in detection of HIV: TB coinfection as HIV positive TB cases are more likely to be smear negative, thus early diagnosis of TB in HIV: TB patients helps in the management of these patients, reducing the morbidity and mortality. In our study out of 69 clinically suspects HIV:TB co-infection cases, one third cases were confirmed by Gene Xpert. Thus, 44 patients were not put on unnecessary AKT and were kept on follow-up. Even though the association of MDR-TB and HIV co-infection was not very significant in this study, it would not be too long before witnessing a rapid increase of MDR-TB among HIV patients if adequate and immediate measures are not taken. In the present study, MDR-TB detection rate was high among retreatment cases. Emphasis has to be given for completion of primary treatment on time and taking proper nutrition. Patients usually stop treatment once they feel better within 2 month of starting the treatment. Special counselling and education is needed at this juncture. In our study, two cases of primary MDR-TB were from household contact. Hence, health workers must generate awareness and educate patient and family members about the risk of acquiring Primary MDR-TB to prevent its spread.

Abbreviations:
MDR-TB: Multidrug resistance tuberculosis
MTB: Mycobacterium tuberculosis
TB: Tuberculosis
RNTCP: Revised National Tuberculosis Programme
DOTS: Directly Observed Therapy, Short-Course
AKT: Anti Koch’s treatment XDR-
TB: Extensively drug resistant tuberculosis
WHO: World Health Organisation HIV: Human Immunodeficiency Virus AIDS: Acquired Immunodeficiency Syndrome

ACKNOWLEDGEMENT
We are thankful to Mr. Gopal Chaure for his technical support. We also extend thanks to RNTCP staff of Dhule district. Authors also acknowledge the immense help received from the scholars whose articles are cited and included in references of this manuscript. The authors are also grateful to authors / editors / publishers of all those articles, journals and books from where the literature for this article has been reviewed and discussed.

References:

1. World Health Organization. Global tuberculosis control: surveillance,planning, financing. WHO report 2008. WHO/ HTM/TB/2008.393. Geneva, Switzerland: WHO, 2008. www. who.int/tb/publications/global_report/2008/en/index.html Accessed June 2009

2. Paramasivan CN. An overview of drug resistant tuberculosis in India. Indian J Tuberc 1998;45:73-81.

3. World Health Organization Report: Global Tuberculosis Control.Surveillance, planning and financing. Geneva, World Health Organization (WHO/HTM/TB/2005.349); 2005.

4. World Health Organization Report: Global Tuberculosis Control.Surveillance, planning and financing. Geneva, World Health Organization (WHO/HTM/TB/2006.362); 2006.

5. World Health Organization Report: Global Tuberculosis Control.Surveillance, planning and financing. Geneva, World Health Organization (WHO/HTM/TB/2007.376); 2007.

6. World Health Organization Report: Global Tuberculosis Control.Surveillance, planning and financing. Geneva, World Health Organization (WHO/HTM/TB/2008.393); 2008.

7. Santha T, Gopi PG, Rajeswari R, Selvakumar N, Subramani R, Chandrasekaran V, Rani B, Thomas A, Narayanan PR: Is it worth treating Category I failure patients with Category I regimen? Ind JTuberc 2005, 52:203-206.

8. Ormerod L.P. Multidrug-resistant tuberculosis (MDR-TB): epidemiology, prevention and treatment. British Medical Bulletin 2005;73 and 74:17–24.

9. Badri M, Wilson D, Wood R. Effect of highly active antiretroviral therapy on incidence of tuberculosis in South Africa: a cohort study. Lancet. 2002;359:2059–64.

10. Cepheid®. Xpert® MTB/RIF Package Insert Ref GXMTB/RIFUS-10. 2015;(Feb).

11. Region SA. Tuberculosis Control in South-East Asia Region. 2015. 435 p.

12. World Health Organization Report: Global Tuberculosis Control.Surveillance, planning and financing. Geneva, World Health Organization (WHO/HTM/TB/2010.7); 2010.

13. Jaiswal RK, Srivastav S, Mahajan H. Socio demographic profile of TB-HIV co-infected patients in Bundelkhand Region, UttarPradesh. Nat J Med Res. 2012;2:149–51.

14. Corbett EL, Watt CJ, Walker N, Maher D, Williams BG, Raviglione MC, et al. The growing burden of tuberculosis: Global trends and interactions with the HIV epidemic. Arch Intern Med. 2003;163:1009–21.

15. Lawn S, Churchyard G. Epidemiology of HIV associated tuberculosis. Curr Opin HIV AIDS.2009;4:325–33.

16. Geneva (Switzerland): Global tuberculosis control: Epidemiology, strategy, financing; 2009. World Health Organization World Health Organization; p. 411. WHO/HTM/TB/2009.

17. Narain JP, Lo YR. Epidemiology of HIV-TB in Asia. Indian J Med Res. 2004;120:277–89.

18. Sethi S, Mewara A, Dhatwalia SK, Singh H, Yadav R, Singh K, et al. Prevalence of multidrugresistance in Mycobacterium tuberculosis isolates from HIV seropositive and seronegative patients with pulmonary tuberculosis in north India. BMC Infect Dis . BioMed Central; 2013 Jan 15 ;13(1):137.

19. Ghiya R, Naik E, Casanas B, Izurieta R, Marfatia Y. Clinico-epidemiological profile of HIV/TB coinfected patients in Vadodara, Gujarat. Indian J Sex Transm Dis . 2009 Jan;30(1):10–5.

20. Noeske J, Dopico E, Torrea G, Wang H, Van Deun A. Two vs. three sputum samples for microscopic detection of tuberculosis in a high HIV prevalence population. Int J Tuberc Lung Dis. 2009;13:842–7.

21. Magana-Arachchi DN. Tuberculosis - Current Issues in Diagnosis and Management [Internet]. Mahboub B, editor. InTech; 2013 [cited 2016 Mar 30]. Available from: http://www. intechopen.com/books/tuberculosis-current-issues-in-diagnosis-and-management/epidemiology-of-multidrug-resistant-tuberculosis-mdr-tb22. WHO 2015. World Health Organization: MDR-TB 2015 Factsheet Source. Available from: http:// www.who.int/tb/challenges/mdr/mdr_tb_factsheet.pdf.

23. MDRTB_Indicators_map [Internet]. [cited 2016 Mar 30]. Available from: https://extranet.who.int/sree/Reports?op=vsandpath=/ WHO_HQ_Reports/G2/PROD/EXT/MDRTB_Indicators_map 24. WHO.Global Tuberculosis Control:WHO Report 2010.2010[Sept18,2011] Available from: http://www.who.int/ tb/publication/global report/2010/en/index.html 25. BrudneyK,Dobkin J. Resurgent tuberculosis in New York City. HIV virus, homeleness and the decline of tuberculosis control programme. Am Rev Respir Dis 1991;144:748-9. 26. Angarno G, Carbonara S,Costd GoriA. Drug resistant TB in HIV infected persons in Italy.Int J Tuberc Lung Dis 1998;2:301- 11. 27 Maranetra KN. Treatment of multidrug-resistant tuberculosis in Thailand. Chemotherapy1996;42:(Suppl 3):10-15. 28. Chiang C-Y, Centis R, Migliori GB. Drug-resistant tuberculosis: past, present, future. Respirology. 2010;15(3):413–32.

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A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh"Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


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International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal

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