International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
Bootstrap Slider

Indexed and Abstracted in: SCOPUS, Crossref, CAS Abstracts, Publons, CiteFactor, Open J-Gate, ROAD, Indian Citation Index (ICI), Indian Journals Index (IJINDEX), Internet Archive, IP Indexing, Google Scholar, Scientific Indexing Services, Index Copernicus, Science Central, Revistas Medicas Portuguesas, EBSCO, BOAI, SOROS, NEWJOUR, ResearchGATE, Ulrich's Periodicals Directory, DocStoc, PdfCast, getCITED, SkyDrive, Citebase, e-Print, WorldCat (World's largest network of library content and services), Electronic Journals Library by University Library of Regensburg, SciPeople.

Search Articles

Track manuscript

Readers around the world

Full Html

IJCRR - Vol 05 Issue 02, January, 2013

Pages: 58-65

Date of Publication: 26-Jan-2013


Print Article   Download XML  Download PDF

ANALYSIS OF Por AND TbpB GENES OF NEISSERIA GONORRHOEAE TO IDENTIFY POTENTIAL MOLECULAR SIGNATURE FOR DETECTING RAPE ACCUSED

Author: Arvind Kumar Rajan, Dhairyawant Kushwaha, Vijay Kumar, Gireesh Babu K.

Category: Healthcare

Abstract:Sexually transmitted infections are a major public health problem. The French laboratories participated on voluntary basis in the RENAGO (R\?seau National du Gonocoque) network and sent all of their collected strains to the National Reference Center for Neisseria gonorrhoeae. Gonorrhoea, caused by the gram-negative bacterium Neisseria gonorrhoeae, remains one of the most common sexually transmitted infections (STIs), causing cervicitis, urethritis, ano-rectitis, and conjunctivitis of the newborn. Over the last few years, the number of gonorrhoea cases has increased regularly in many European countries (Fenton, K. A., and C. M. Lowndes. 2004). Since the first report of tetracycline resistance in 1985, gonococci that are resistant to tetracycline have spread globally, coexisting chromosomally and plasmid-mediated resistanc isolates (Starnino, S., A. Neri, and P. Stefanelli. 2008.). At present, there is no effective vaccine against N. gonorrhoeae. The control of gonococcal infections depends on in pursuing of our populations at risk, on public health measures to limit the spread of infection, and on early intervention to treat infected individuals. Any kind of methods have been used for molecular epidemiology studies of N. gonorrhoea. Some of these methods are based on growth requirements for specific nutrients, antibiotic susceptibility, differences in multilocus enzyme electrophoresis, and serological reactivity against surface antigens. Because the above approaches present several limitations, molecular typing methods have been developed with improved discrimination among gonococcal isolates. Of the currently available sequence-based methods, N. gonorrhoeae multiantigen sequence typing (NG-MAST) is one of the most useful because it generates a simple numerical sequence type (ST) based on the combined sequences of two genes (por and tbpB). In addition, an internationally accessible web database allows strain comparison worldwide (Martin, I. M., S. Hoffmann, and C. A. Ison 2006).

Full Text:

INTRODUCTION

A crime that typically produces physical evidence. Often, only the victim and the perpetrator are present when the crime occurs, so this evidence is critical. Sexual assault includes rape, both heterosexual and homosexual, child rape and abuse, and elder abuse. Sexual assault can also be part of other crimes such as burglary and murder. The key points of evidence collection are at the scene and also at the hospital or other medical facility where the victim is examined. This collection can also occur during autopsy. In either case, sexual assault evidence collected by medical personnel centers on the sexual assault kit, which consists of blood, body fluids (principally semen and saliva), and many other items. Trace evidence can be significant as well since an assault try to edit lends itself to rich transfer of fibers, broken fingernails, and forcibly removed hair, for example are playing an increasingly important role in sexual assault cases.

There are also many advantages to using DNA fingerprinting in sexual assault or rape cases. DNA fingerprinting is accurate at identifying a rapist based on evidence left behind on the victim. Detectives can simply match the DNA of the specimen found at the crime scene/ after the crime time, with the DNA of the alleged suspect determine who is guilty of the crime. DNA samples from the alleged rapist can be collected through a vaginal swab from the victim or on other semen found in the scene or from the clothing. By applying molecular-biology methods, DNA profiling uses repetitive ("repeat") sequences that are highly variable (Accessexcellence.org) called variable number tandem repeats (VNTRs), particularly short tandem repeats (STRs). VNTR loci are very similar between closely related DNA samples, but so variable that unrelated individuals are extremely unlikely to have the same VNTRs. Besides these repeats PCR-RFLP might also be useful for the aforesaid purpose. Sexual assault is a violent crime that affects men, women, and children of all ages. Sexually transmitted diseases (STDs) may be transmitted during sexual assault. In children, isolation of sexually transmitted organism may be the first indication that abuse has occurred. The bacterium Neisseria gonorrhoeae causes gonorrhoea, a disease which is almost exclusively transmitted during sexual intercourse. Currently, isolation and identification of the causative organism remains the gold standard for a definitive diagnosis. Although tests requiring cultures of the bacterium are considered to be best for a definative diagnosis, molecular methods relying on only bacterial DNA are improving in sensitivity and specificity. However, nucleic-acid based techniques are being used more frequently as sensitivity and specificity of the newer tests are improved (Palmer et al., 2003), although none have been licensed for use with specimens from non-genital sites, including the rectum and the pharynx. There is also little validation of molecular methods for diagnosis of gonorrhoea in children and they should be used with caution as the prevalence of infection is likely to be low (Hammerschlag, 2001).These can permit detection and typing of N. gonorrhoeae in situations where the bacterium cannot be cultured. For medico-legal cases there is a need to not only confirm the presence of N. gonorrhoeae, but to prove a link, or the absence of one, between the strains isolated from each individual. DNA markers therefore need be selected so that they accumulate variation sufficiently rapidly to exclude unlinked individuals who happen to suffer from gonorrhoea, while still allowing identification of individuals who are sexual contacts or part of a short transmission chain. A database of suitable DNA markers for N. gonorrhoeae acceptable in a court of law is not yet available. However, researchers have a database from two of the bacterium’s most variable genes containing information on over 4,000 different strains. This is the N. gonorrhoeae multi-antigen sequence typing database (NG-MAST), but since the strains were collected during specific studies, they do not give a complete picture of all the types present within a particular geographical area. However, researchers were recently faced with a request for N. gonorrhoeae confirmation that utilized NGMAST, and provided the first reported typing of gonococcal DNA from clothing for medico-legal reasons. In medico-legal cases, such as sexual abuse of a child or rape, it is necessary not only to confirm the presence of the infecting organism but also to attempt to prove linkage between the isolates from each individual involved. Typing of gonococcal isolates has been used try to edit because it can support or dispute the likelihood of transmission between individuals, and newer molecular methods offer greater discrimination compared to phenotypic methods (Ison, 1998). A genotypic method needs to be based on a target that accumulates genetic variation quickly so individuals that are sexual contacts or part of a short transmission chain should demonstrate an indistinguishable type, while individuals that are unlinked should have different types. N. gonorrhoeae multi-antigen sequence typing (NGMAST) is a sequence-based method examining the diversity in two outer-membrane proteins, Por and TbpB (transferrin binding protein B), allowing the precise characterization and comparison of gonococcal samples, with all analysis performed via a website (http://www.ngmast.net). The method has been shown to identify epidemiologically linked individuals and distinguish between individuals that are not linked (Martin et al., 2004). Ideally, variation in hyper-variable genes, resulting from genetic exchange and recombination during mixed infection, should produce a changing population of molecular types over time. Highly diverse populations have been identified (Choudhury et al., 2006) but persistence of molecular types within a population has also been shown (Ward et al., 2000) and may reflect a lower rate of partner change and hence chance of mixed infection. The Sexually Transmitted Bacteria Reference Laboratory (STBRL) at the Health Protection Agency Centre for Infections uses molecular methods for confirmation of identity and typing when isolates are received from potentially linked sources, to support or dispute the likelihood of transmission of gonorrhoea. This short report describes the first use of molecular methods to confirm the presence of N. gonorrhoeae from adult clothing and provide evidence of possible linkage to an isolate from a child (Martin et al. 2007). Since there are major reports on the presence of molecular candidate markers which can detect or discriminate the rape accused from rape victims while using the sexually transmitted agents, the present study is designed with the following

• Retrieval of Por and TbpB genes sequences of Nesseria gonohorroea strains. Identification of DNA based signature by comparative in-silico analysis of retrieved Por and TbpB genes sequences.

• In silico validation of selected DNA signatures.

MATERIAL AND METHOD
N. gonorrhoeae isolates.

A first group of 67 isolates (group I) was selected from different antibiotic resistance phenotypes and then from different geographic origins, from male and female patients, and finally from different sampling locations (cervix, urethra, urine, and knee fluid), identify possible clusters. A second group of 26 isolates (group II) was selected from rectal samples from men who have sex with men (MSM), without of antibiotic phenotype restriction criteria, in order to identify specific clusters in this high-risk population.

Culture and identification

The isolates were cultured on PVX chocolate agar (bioMérieux, Marcy-l'Étoile, France) supplemented with 1% IsoVitaleX and incubated overnight at 37°C with 5% carbon dioxide. Identification was performed with the API NH system (bioMérieux, Marcy-l'Étoile, France).

Phenotypic analysis

Isolates were tested by routine analysis for antibiotic susceptibility and serotype. Susceptibility to six antibiotics (penicillin, ceftriaxone, tetracycline, ciprofloxacin, spectinomycin, and erythromycin) was measured using the disk diffusion method (bioMérieux, Marcy-l'Étoile, France). β-Lactamase production was detected by the cefinase test (bioMérieux, Marcy-l'Étoile, France). Etests (AB Biodisk, Sweden) were used to determine MICs of penicillin, ceftriaxone, tetracycline, ciprofloxacin, and spectinomycin. Results were interpreted according to the Clinical and Laboratory

Standards Institute (CLSI). Isolates were serotyped with the Phadebact GC serovar test using monoclonal antibodies (Orgentec SAS, Trappes, France).

DNA extraction
Isolates were retrieved from storage at −80°C in glycerol broth, cultured on PVX chocolate agar supplemented with 1% IsoVitaleX, and incubated overnight at 37°C with 5% carbon dioxide. A turbid suspension of the gonococcal subculture was made in phosphate-buffered saline (optical density of ~2.0). The bacteria were pelleted by centrifugation at 2,000 × g for 5 min, washed once, resuspended in phosphate-buffered saline, and boiled for 5 min to lyse the cells. The lysate was centrifuged for 5 min at 2,000 × g, and the supernatant was stored at −20°C until required.

Molecular typing by NG-MAST
Briefly, internal regions of the por and tbpB genes were amplified by PCR from the DNA extracts, and both strands were sequenced using with Big Dye v.1.1 chemistry on an ABI3730XL capillary sequencer (Applied BioSystems). Sequences were aligned, edited, and trimmed to a fixed length from conserved positions as described previously (Martin, I. M., S. Hoffmann, and C. A. Ison. 2006.) using BioNumerics software (Applied Maths, Belgium). Alleles were assigned to each sequence variant of por and tbpB, and the corresponding ST was assigned through the NG-MAST website (http://www.ngmast.net) based on the combination of the alleles of the two loci.

RESULTS

The 67 group I isolates (from a heterosexual population and various geographic and clinical sources) came from India and its surroundings (25 isolates [37%]) and from other regions (42 isolates [63%]). Sixty-one isolates were from males and 6 from women. This low number of isolates from women could be explained by the asymptomatic or paucisymptomatic gonococcal infections observed in women who did not attend an STI clinic. The diagnosis of N. gonorrhoeae infection is more often casual, generally a gynecological checkup carriage in women is characterized by a low number of bacteria, which are particularly difficult to isolate, among the commensal vaginal bacteria. The ages were known for 56 men (mean, 32 years; range, 18 to 57 years) and 5 women (mean, 31 years; range, 18 to 62 years) and unknown for 6 other patients (5 men and 1 woman). Fifty-six isolates were obtained from male urethra, 6 from cervix, 4 from male urine, and 1 from the fluid of a male's knee. The last isolate was included because it was collected from an exceptional location and characterized by the rare antibiotic resistance phenotype 2. The majority of the group II isolates (MSM) came from India and its surrounding area (88%), with an average patient age of 33 years (range, 20 to 54 years). Based on the MICs obtained for penicillin (with or without β-lactamase production), tetracycline, ciprofloxacin, ceftriaxone, and spectinomycin, the 93 isolates could be classified into six phenotypes representing an overview of the diversity of antibiotic resistance phenotypes observed in France. Thirty-nine (42%) isolates had antibiotic susceptibility phenotype 3, characterized by a susceptibility to all tested antibiotics, whereas 31 (33%) isolates had phenotype 4 (with reduced susceptibility to ceftriaxone and high-level resistance to ciprofloxacin). The remaining isolates were divided into the four other susceptibility phenotypes, which was distinguish by their combination of resistance to tetracycline, ciprofloxacin, and ceftriaxone. Based on NG-MAST, the 93 isolates were subdivided into 53 different STs, including previously described STs as well as new STs. Most STs (75%) were only represented by single isolate, whereas the remaining STs were represented by 2 to 16 isolates. The 13 most common STs were ST225 (16 isolates [17.2%]);

ST1479 (9 isolates [9.7%]); ST880 (4 isolates [4.3%]); ST26, ST437, ST975, and ST1986 (3 isolates [3.2%]); and ST2, ST211, ST292, ST359, ST1813, and ST1987 (2 isolates [2.1%]). Among the 67 group I isolates, we could identify 36 different STs, 10 of which included more than one isolate (ST225, ST1479, ST26, ST975, ST437, ST1986, ST2, ST1987, ST211, and ST880), together clustering 61% of the isolates.Regarding the 26 isolates from group II, six STs were found with more than one isolate (ST225, ST880, ST292, ST359 and ST1813), grouping 38% of the isolates. The diversity indices were similar in the two groups (Simpson's index, 92.3% versus 94.7%, respectively). Fourteen isolates from group I and two isolates from group II belonged to ST225, among which 14 isolates were characterized by antibiotic resistance phenotype 4. ST1479 grouped eight isolates from different geographic regions from group I and one isolate from group II. The eight isolates presented the two similar antibiotic resistance phenotypes 1 and 2 (differing by the presence or absence of a β-lactamase). Of note, the isolate from knee fluid was ST1479. ST880 was represented by two isolates from group I and two isolates from group II, all of which exhibited antibiotic phenotype 4. Antibiotic resistance phenotype 5 (with a reduced susceptibility to ceftriaxone) was observed only in two isolates from group I (ST21 and ST1985). These STs were closely related, since they share the same tbpB allele (allele 33) and their porB alleles differed by two single nucleotide polymorphisms (allele 14 for ST21 and allele 467 for ST1985). Antibiotic phenotype 6 (resistance to tetracycline and ciprofloxacin) was observed only in group II, in five isolates of distinct STs. Notably, the genetic diversity differed according to the susceptibility phenotype of the isolates. Isolates with antibiotic phenotype 3 (susceptible to all tested antibiotics) were associated with many single STs, whereas a majority (22/31 [71%]) of isolates with antibiotic phenotype 4 (with reduced susceptibility to ceftriaxone and high resistance to ciprofloxacin) were clustered in the STs ST225, ST880, and ST437. In addition, these three STs were closely related, as the allele sequences of tbpB gene were identical (allele 4). Moreover, the por allele of ST225 (allele 4) differed from that of ST437 (allele 14) and ST880 (allele 489) by a single nucleotide polymorphism. These observations indicate a common ancestral origin of these isolates, from which they acquired their phenotype.

CONCLUSION

Finding the fact in crimes against physical integrity is not possible without using forensic techniques. In order to find the fact in every case, there is a set of methods which are usually inter related. Forensic sciences can be used in three main stages; first the establishment of the criminal conduct and its effects, second the determination of the offender and third identification of the victim. The development of scientific means and testing in the context of crime detection has led to a higher quality of forensic techniques; DNA testing is a good example in this respect. However Forensic experts should take into account every trivial thing that may be relevant in the course of the criminal conduct. An examination after rape requires the use of a reliable semen detection test that is sensitive and has very good negative predictive power. Previous studies have shown that cytology allows the detection of some but not all types of sperm up to 72 h whereas a search for acid phosphatases does detect all types of sperm but only in the first 24 h. Regarding the PSA detection kit, it allowed all types of semen to be detected up to 48 h (Schieferstein 1999). The commercialization of rapid detection kits would make the routine search for PSA more viable. There is an excellent concordance between the PSA-Check 1® kit and the spermogram, with very good negative predictive values even at 48 h. In our opinion, the PSA test is the best marker of the presence of semen and is well suited for use in emergency consultations. If necessary, a cytological test could be performed during the consultation if the PSA result were to be negative after 48 h. Rape is indexed as the second-most-frequent violent crime, yet it also is considered the crime that is least reported. Rape is a serious offense against the person, assaulting both body and psyche, and victims may suffer short- or longterm impairment as well. As primary care providers, nurse practitioners are in a position to identify rape victims and begin interventions to alleviate suffering, provide supportive care and plan for follow-up in order to prevent devastating effects on the individual's life. The purpose of this article is to better acquaint nurse practitioners with the scope of rape and rape issues, and the impact rape has on adult female victims and their families. The article discusses the definition of rape; rape myths; prevalence and reporting; responses of practitioners, victims and families; and primary care interventions in the areas of counseling, physical assessment and treatment, and police/legal considerations. And suggested some molecular typing methods to detect presence of N. gonorrheae which is a sexually transmitted disease implanted by accused in victims.

References:

1. Choudhury, B., Risley, C. L., Ghani, A. C., Bishop, C. J., Ward, H., Fenton, K. A., Ison, C. A. & Spratt, B. G. (2006). Identification of individuals with gonorrhoea within sexual networks: a population based study. Lancet 368, 139–146.

2. Fenton, K. A., and C. M. Lowndes. 2004. Recent trends in the epidemiology of sexually transmitted infections in the European Union. Sex. Transm. Infect. 80:255-263. [http://www.ncbi.nlm.nih.gov/pmc/articles/P MC1744866/

3. Gallay, A., A. Bouyssou-Michel, F. Lassau, B. Basselier, P. Sednaoui, and Laboratoires du Réseau Renago. 2008. Neisseria gonorrhoeae infections in France in 2006: significant progression in women and persistent increase of ciprofloxacin resistance. Bull. Epidémiol. Hebdomadaire 5- 6:33-36. (In French.)

4. Hanage, W. P., Fraser, C. & Spratt, B. G. (2005). Fuzzy species among recombinogenic bacteria. BMC Biol 3, 6–13.

5. Ison, C. A. (1998). Genotyping of N. gonorrhoeae. Curr Opin Infect Dis 11, 43–46.

6. Ison, C., C. M. Bellinger, and J. Walker. 1986. Homology of cryptic plasmid of Neisseria gonorrhoeae with plasmids from Neisseria meningitidis and Neisseria lactamica. J. Clin. Pathol. 39:1119–1123.

7. Jenny, C. (1992). Sexually transmitted diseases and child abuse. Pediatr Ann 21, 497–503.

8. Liao, M., K. Bell, W. M. Gu, Y. Yang, N. F. Eng, W. Fu, L. Wu, C. G. Zhang, Y. Chen, A. M. Jolly, and J. A. Dillon. 2008. Clusters of circulating Neisseria gonorrhoeae strains and association with antimicrobial resistance in Shanghai. J. Antimicrob. Chemother. 61:478-487.

9. Lum, G., Freeman, K., Nguyen, N. L., Limnios, E. A., Tabrizi, S. N., Carter, I., Chambers, I. W., Whiley, D. M., Sloots, T. P.& other authors (2005). A cluster of culture positive gonococcal infections but with false negative cppB gene based PCR. Sex Transm Infect 81, 400–402.

10. Martin, I. M., S. Hoffmann, and C. A. Ison. 2006. European Surveillance of Sexually Transmitted Infections (ESSTI): the first combined antimicrobial susceptibility data for Neisseria gonorrhoeae in Western Europe. J. Antimicrob. Chemother. 58:587-593

11. Martin I. M. C., Ellie Foreman, Vicky Hall, Anne Nesbitt, Greta Forster and Catherine A. Ison (2007). Non-cultural detection and molecular genotyping of Neisseria gonorrhoeae from a piece of clothing. Journal of Medical Microbiology, 56, 487–490.

12. Martin, I. M. C., Ison, C. A., Aanensen, D. M., Fenton, K. A. & Spratt, B. G. (2004). Rapid sequence-based identification of gonococcal transmission clusters in a large metropolitan area. J Infect Dis 189, 1497– 1505.

13. Newman, L. M., J. S. Moran, and K. A. Workowski. 2007. Update on the management of gonorrhea in adults in the United States. Clin. Infect. Dis. 44(Suppl. 3):S84-S101.

14. Neinstein, L. S., Goldenring, J. & Carpenter, S. (1984). Nonsexual transmission of sexually transmitted diseases: an infrequent occurrence. Pediatrics 74, 67–76.

15. Perez-Losada, M., K. A. Crandall, J. Zenilman, and R. P. Viscidi. 2007. Temporal trends in gonococcal population genetics in a high prevalence urban community. Infect. Genet. Evol. 7:271-278.

16. Palmer, H. M., Mallinson, H., Wood, R. L. & Herring, A. J. (2003). Evaluation of the specificities of five DNA amplification methods for the detection of Neisseria gonorrhoeae. J Clin Microbiol 41, 835–837.

17. Starnino, S., A. Neri, and P. Stefanelli. 2008. Molecular analysis of tetracycline-resistant gonococci: rapid detection of resistant genotypes using a real-time PCR assay. FEMS Microbiol. Lett. 286:16-23.

18. Smith, D. W., Tapsall, J. W. & Lum, G. (2005). Guidelines for the use and interpretation of nucleic acid detection tests for Neisseria gonorrhoeae in Australia: a position paper on behalf of the Public Health Laboratory Network. Commun Dis Intell 29, 358–365.

19. Tabrizi, S. N., S. Chen, M. A. Cohenford, B. B. Lentrichia, E. Coffman, T. Schultz, J. W. Tapsall, and S. M. Garland. 2004. Evaluation of real time polymerase chain reaction assays for confirmation of Neisseria gonorrhoeae in clinical samples tested positive in the Roche Cobas Amplicor assay. Sex. Transm. Infect. 80:68–71.

20. Use of DNA in Identification. Accessexcellence.org. Retrieved 2010-04-03.

21. Ward, H., Ison, C. A., Day, S. E., Martin, I. M., Ghani, A. C., Garnett, G. P., Bell, G., Kinghorn, G. & Weber, J. N. A. (2000). A prospective social and molecularinvestigation of gonococcal transmission. Lancet 356, 1812–1817.

22. Whiley, D. M., J. W. Tapsall, and T. P. Sloots. 2006. Nucleic acid amplification testing for Neisseria gonorrhoeae. An ongoing challenge. J. Mol. Diagn. 8:3–14.

23. Whiley, D. M., S. M. Garland, G. Harnett, G. Lum, D. W. Smith, S. N. Tabrizi, T. P. Sloots, and J. W. Tapsall. 2008. Exploring “best practice” for nucleic acid detection of Neisseria gonorrhoeae. Sex. Health 5:12–23.

24. Whiley, D. M., Tapsall, J. W. & Sloots, T. P. (2006). Nucleic acid amplification testing for Neisseria gonorrhoeae: an ongoing challenge. J Mol Diagn 8, 3–15.

Research Incentive Schemes

Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award: 

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to editor@ijcrr.com for more details.

Women Researcher Award:

This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.


Best Article Award

A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by Leow Jun Xian and Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh et al. entitled "Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma COLAKOGLU entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by Arpita M. et al entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09
Late to bed everyday? You may die early, get depression
Egg a day tied to lower risk of heart disease
88 Percent Of Delhi Population Has Vitamin D Deficiency: ASSOCHAM Report

List of Awardees

Awardees of COVID-19 Research

Woman Researcher Award

A Study by Neha Garg et al. entitled "Optimization of the Response to nCOVID-19 Pandemic in Pregnant Women – An Urgent Appeal in Indian Scenario" published in Vol 12 issue 09

A Study by Sana Parveen and Shraddha Jain entitled "Pathophysiologic Enigma of COVID-19 Pandemic with Clinical Correlates" published in Vol 12 issue 13

A Study by Rashmi Jain et al. entitled "Current Consensus Review Article on Drugs and Biologics against nCOVID-19 – A Systematic Review" published in Vol 12 issue 09

Emerging Researcher Award

A Study by Madhan Jeyaraman et al. entitled "Vitamin-D: An Immune Shield Against nCOVID-19" published in Vol 12 issue 09

Study by Dheeraj Kumar Chopra et al. entitled "Lipid-Based Solid Dispersions of Azilsartan Medoxomil with Improved Oral Bioavailability: In Vitro and In Vivo Evaluation" published in Vol 12 issue 19


RSS feed

Indexed and Abstracted in


Antiplagiarism Policy: IJCRR strongly condemn and discourage practice of plagiarism. All received manuscripts have to pass through "Plagiarism Detection Software" test before forwarding for peer review. We consider "Plagiarism is a crime"

IJCRR Code of Conduct: We at IJCRR voluntarily adopt policies on Code of Conduct, and Code of Ethics given by OASPA and COPE. To know about IJCRRs Code of Conduct, Code of Ethics, Artical Retraction policy, Digital Preservation Policy, and Journals Licence policy click here

Disclaimer: International Journal of Current Research and Review (JICRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal.



Company name

International Journal of Current Research and Review (JICRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal

Contact

148, IMSR Building, Ayurvedic Layout,
        Near NIT Complex, Sakkardara,
        Nagpur-24, Maharashtra State, India

editor@ijcrr.com

editor.ijcrr@gmail.com


Copyright © 2021 IJCRR. Specialized online journals by ubijournal .Website by Ubitech solutions