International Journal of Current Research and Review
ISSN: 2231-2196 (Print)ISSN: 0975-5241 (Online)
logo
slider
slider
slider
slider
Bootstrap Slider

Indexed and Abstracted in: Crossref, CAS Abstracts, Publons, Google Scholar, Open J-Gate, ROAD, Indian Citation Index (ICI), ResearchGATE, Ulrich's Periodicals Directory, WorldCat (World's largest network of library content and services)

Search Articles

Track manuscript

Full Html

IJCRR - 5(7), April, 2013

Pages: 17-24

Date of Publication: 18-Apr-2013


Print Article   Download XML  Download PDF

INVESTIGATION OF ANTI BACTERIAL AND ANTI FUGAL POTENTIALS OF MACARANGA PELTATA

Author: Meenakshi Verma, Narayanan K., Mitali Thakar B., Subrahmanyam V.M., Venkata Rao J., Dhanaraj S.A., Vasanth Raj P.

Category: General Sciences

Abstract:Macaranga peltata belonging to Euphorbiaceae family. Leaves and stem bark were separated, shade dried and extracted separately using methanol. The concentrated methanolic extracts of Macaranga peltata were subjected to antimicrobial studies. The percentage yield of the leaves and stem bark were found to be 47% and 30% respectively. The anti-bacterial activity of Leaf and Stem Bark extracts of Macaranga peltata was determined by Cup-plate method and Zone of Inhibition was measured. Zone of Inhibition was found to be 16mm for Leaf extract and 13mm and 14mm for Stem Bark extract in two different sets against Escherichia coli. Out of two Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus Zone of Inhibition was obtained only for Staphylococcus aureus that was 16mm for Leaf extract and 12mm and 13mm for Stem Bark. Leaf extracts showed better anti-bacterial activity than the Stem Bark extract against both Gram-positive and gram-negative bacteria. Minimum Inhibitory concentration (MIC) for Leaf extract was between 62.5\?g/ml to 125\?g/ml for Escherichia coli, 125\?g/ml to 250\?g/ml for Pseudomonas aeruginosa, 62.5\?g/ml to 125\?g/ml for Bacillus subtilis and 31.25\?g/ml to 62.5\?g/ml for Staphylococcus aureus. Whereas MIC for Stem Bark extract was between 500\?g/ml to 1000\?g/ml for Escherichia coli, 250\?g/ml to 500\?g/ml for Pseudomonas aeruginosa, 62.5\?g/ml to 125\?g/ml for Bacillus subtilis and 62.5\?g/ml to 125\?g/ml for Staphylococcus aureus. Ciprofloxacin used as standard antibiotic showed MIC between 1.953 \?g/ml to7.813 \?g/ml for all four bacterial strains.

Keywords: Macaranga peltata, Euphorbiaceae, anti-bacterial and Cup-plate method

Full Text:

INTRODUCTION

The plant is a biosynthetic laboratory, not only for chemical compounds, but also a multitude of compounds like glycosides, alkaloids etc. These exert physiological and therapeutic effect. The compounds that are responsible for medicinal property of the drug are usually secondary metabolites. A systematic study of a crude drug embraces through consideration of primary and secondary metabolites derived as a result of plant metabolism. The plant material is subjected to phytochemical screening for the detection of various plant constituents [1]. With onset of scientific research in herbals, it is becoming clearer that the medicinal herbs have a potential in today’s synthetic era, as numbers of medicines are becoming resistant. According to one estimate only 20% of the plant flora has been studied and 60%of synthetic medicines owe their origin to plants. Ancient knowledge coupled with scientific principles can come to the forefront and provide us with powerful remedies to eradicate the diseases.
According to World Health Organization (WHO) more than 80% of the world's population relies on traditional medicine for their primary healthcare needs. Plants used for traditional medicine contain a wide range of substances that can be used to treat chronic as well as infectious diseases [2]. The 30th World Health Assembly adopted a resolution urging interested governments to utilize their traditional systems of medicine with regulations suited to their national health care systems [3]. Utilization of plants for medicinal purposes has been documented long back in ancient literature. However, organized studies in this direction were initiated in 1956 and off late such studies are gaining recognition and popularity due to loss of traditional knowledge and declining plant population [4]. Macaranga peltata is one of important plants widely found in Western Ghats in India. Macaranga peltata belonging to Euphorbiaceae family is a genus of important pioneer trees widely distributed in south east Asia. Macaranga is a dicot plant with more than 350 species. Genus Macaranga is native to Africa, Australia, Asia and the South Pacific. There are evidences that show that bergenin derivatives and polyphenols have been recorded from Macanga peltata and other flavonoids as well as diterpinoids are suspected to be present. A previous study on Macaranga genus includes study of Macaranga alnifolia for cytotoxic natural products from tropical rainforests in Madagascar through the International Cooperative Biodiversity Group (ICBG) program. Ethanolic extract of the fruit of Macaranga alnifolia Baker (Euphorbiaceae) was obtained for phytochemical investigation. This extract was found to be active in the A2780 ovarian cancer cytotoxicity assay. Bioassayguided fractionation led to the isolation of the five new compounds [5]. Although several traditional plant extracts have historically been known to have antimicrobial activity, to date, there has been relatively little or in some cases, no reports examining the activity against several medically important bacterial and fungal pathogens [6]. Considering the vast of potentiality of plants as sources for antimicrobial drugs with reference to antibacterial and antifungal agents, a systematic investigation was undertaken to screen the local flora for antibacterial and antifungal activity from Macanga peltata.

MATERIALS AND METHODS MATERIALS

All solvents and reagents purchased form Merck Pvt. Ltd, Mumbai, India. All media components purchased from Himedia (India). All the microorganisms were procured from National Collection of Industrial Microorganisms (NCIM), Pune and subcultured in the laboratory.

METHODS

Collection and Authentication of Plant
The plant Macaranga peltata was collected from the Syndicate circle, Manipal in the month of January 2009. The plant was authenticated by Dr. Chandrakant Bhat, Professor, Muniyal Ayurvedic College, Manipal, Karnataka India.

Preparation of Extracts:
The collected plant parts (leaves and stem bark) were separated and shade dried. Then the dried parts were chopped and coarsely powdered; the powdered plant materials were extracted separately using methanol by soxhlet extraction. The amount of methanol was taken as methanol : dry powder (100:15) The extracts were then concentrated to dryness under reduced pressure and controlled temperature to yield a deep brown – dark brown semisolids, the percentage yields was calculated.. All the extracts were stored in refrigerator till further use.

Phytochemical Studies: Qualitative Phytochemical Analysis:
The concentrated methanolic extracts of Macaranga peltata were subjected to chemical test as per the standard methods for the identification of the various constituents. 2-3 mL of the extract solution was taken for all the tests for viz., carbohydrates, Alkaloids, Steroids and Sterols, Glycosides, Saponins, Flavanoids, Tannins, Triterpenoids, Protein and Amino acid:[7] (results not shown).

Antimicrobial activity [8]:
(a) Growth and Maintenance of Test Microorganism for Antimicrobial Studies:

Bacillus subtilis – NCIM 2063 Escherichia coli – NCIM 5011 Pseudomonas aeruginosa – NCIM 7312 Staphylococcus aureus – NCIM 2079 Aspergillus niger – NCIM 545 Candida albicans – NCIM 3100 All the microorganisms were procured from National Collection of Industrial Microorganisms (NCIM), Pune and subcultured in the laboratory. The bacterial cultures were maintained in slants till further use and fungus cultures were maintained on Sabraud’s dextrose agar (SDA).
(b) Cup Plate method for determination of Zone of Inhibition: Nutrient agar (20ml) was prepared according to the manufacturers’ recommendation and sterilized. Plates containing 20 ml of sterile nutrient agar each were inoculated with standardized innocula using sterile Pasteur pipette. Six wells each of 5 mm diameter were made on each plate-1 for antibiotic (Ciprofloxacin for antibacterial), 1 for DMSO (Solvent control), 2 for Leaf Extract and 2 for Stem Bark Extract. 50µl of the plant extracts, antibiotic and DMSO were dispensed into subsequent wells. The extracts were allowed to diffuse into the medium for 1hr at room temperature. This was then incubated at for 24 h at 37ºC after which the zones of growth inhibition were measured and recorded in millimeter [9].
(c) Determination of Minimum Inhibitory Concentration by Tube Dilution method: MIC is the lowest concentration of the drug in which turbidity/growth is not observed. A series of test tubes were prepared containing the same volume of medium inoculated with the test organism to obtain about 104 CFU/ml. Two fold serial dilutions were carried out using 1.8 ml of the above inoculum and 0.2 ml test solution. Positive control containing antibiotic, negative control containing uninoculated broth and solvent control containing DMSO were also used. The tubes were incubated at 37º C for 18 hours (Bacteria) or 28º C for 40 hours (Fungi) [10]. The tubes are inspected visually to determine whether the microorganism has grown, as indicated by turbidity. Tubes in which the extract/ antibiotic is present in a concentration sufficient to inhibit bacterial growth remained clear.

Yield of plant extract:
Percentage yield obtained from leaf was 47% and from stem bark powder was 30% that is very good yield (Table no.1).

Antimicrobial activity:
Standardization of Colonies:

Two Gram-positive and two Gram-negative bacterial colonies were standardized and results were obtained. The numbers of bacterial colonies were represented in terms of Colony Forming Unit (Table no.2).

Antimicrobial Activity (Zone of Inhibition):
Anti-microbial activity of the plant extracts were determined by Cup-Plate Method and activity was measured by measuring Zone of Inhibition in millimeters. Results were obtained for each bacterial strain tested against Macaranga peltata Leaf and Stem Bark extract. Anti-bacterial activity was obtained only for one Gram-positive and one Gram-negative bacteria out of two gram-positive and two Gram-negative bacterial strains. Leaf extract showed better activity against both Gram-positive and Gramnegative bacteria. DMSO used as solvent control, did not inhibit growth of bacteria. Activity of the extracts was found to be less than the standard antibiotic Ciprofloxacin (Table no.3).

RESULTS

Minimum Inhibitory concentration (MIC): The results for MIC of Macaranga peltata observed against different bacterial species were recorded.

MIC for E.coli:
Very low concentration i.e. between 3.906 µg/ml to7.813 µg/ml of ciprofloxacin (used as standard anti-biotic) was able to inhibit the bacterial growth. Leaf extract inhibited the bacterial growth between 62.5µg/ml to 125µg/ml concentration while Stem Bark extract inhibited growth of bacteria between 500µg/ml to 1000µg/ml of concentration. DMSO at its higher concentrations was found to inhibit bacterial growth. Although activity showed by plant extracts was lesser as compared to standard, still Leaf extract showed better activity than Stem Bark extracts (Table no.4).

MIC for Pseudomonas:
Very low concentration i.e. between 1.953 µg/ml to 3.906 µg/ml of ciprofloxacin (used as standard anti-biotic) was able to inhibit the bacterial growth. Leaf extract inhibited the bacterial growth between 125µg/ml to 250µg/ml concentration while Stem Bark extract inhibited growth of bacteria between 250µg/ml to 500µg/ml of concentration. DMSO at its higher concentrations was found to inhibit bacterial growth. Although activity showed by plant extracts was lesser as compared to standard, still Leaf extract showed better activity than Stem Bark extracts (Table no.5).

MIC for Bacillus:
Very low concentration i.e. between 1.953 µg/ml to 3.906 µg/ml of ciprofloxacin (used as standard anti-biotic) was able to inhibit the bacterial growth. Both Leaf extract and Stem Bark extracts inhibited the bacterial growth between 62.5µg/ml to 125µg/ml concentration (Table no.6). DMSO at its higher concentrations was found to inhibit bacterial growth. Although activity showed by plant extracts was less, still it was comparable with standard.

MIC for Staphylococcus:
Very low concentration i.e. between 1.953 µg/ml to 3.906 µg/ml of ciprofloxacin (used as standard antibiotic) was able to inhibit the bacterial growth. Leaf extract inhibited the bacterial growth between 31.25µg/ml to 62.5µg/ml concentration while Stem Bark extract inhibited growth of bacteria between 62.5µg/ml to 125µg/ml of concentration. DMSO at its higher concentrations was found to inhibit bacterial growth. Although activity showed by plant extracts was lesser as compared to standard, still Leaf extract showed better activity than Stem Bark extracts (Table no.7).

DISCUSSION

Macaranga peltata belonging to Euphorbiaceae family is one of the important plants found in Western ghats specially in Udupi district, which contains potential for active compounds is very less studied. Since different species of Macaranga i.e. Macaranga alnifolia has been already studied for its anti-proliferative activity, Macaranga peltata is studied for its antimicrobial activity. Leaf and Stem Bark samples were collected from Macaranga peltata and shade dried and powdered. Methanolic extracts of these samples were obtained by Soxhlet extraction method. The yield obtained from leaf was 47% and from Stem Bark was 30%. Both plant extracts were subjected to phytochemical analysis. Leaf extract showed the presence of carbohydrates, steroids and sterols, glycisides, flavonoids, tannins, proteins and amino acids while Stem Bark extract showed positive results for carbohydrates, glycosides, saponins, flavonoids, tannins, proteins and amino acids. Biological studies of plant extracts indicate anti-microbial activity. Anti-microbial activity was performed in two steps, one for anti-bacterial and another for antifungal. To screen the plant Macaranga peltata for anti-bacterial activity four bacterial strains were selected; two Gram-positive (Bacillus subtilis and staphylococcus aureus) and two Gram-negative (Escherichia coli and Pseudomonas aeruginosa). These bacterial strains were maintained in slants containing Nutrient Agar and sub-cultured in nutrient broth during use. The standardization of each strain was done and Colony Forming Unit (CFU) for each was determined. The anti-bacterial activity of Leaf and Stem Bark extracts of Macaranga peltata was determined by Cup-plate method and Zone of Inhibition was measured. Zone of Inhibition was found to be 16mm for Leaf extract and 13mm and 14mm for Stem Bark extract in two different sets against Escherichia coli while there was no inhibition observed against another Gram-negative strain Pseudomonas aeruginosa. Out of two Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus Zone of Inhibition was obtained only for Staphylococcus aureus that was 16mm for Leaf extract and 12mm and 13mm for Stem Bark extract in two different sets of the experiment. Leaf extracts showed better anti-bacterial activity than the Stem Bark extract against both Gram-positive and gram-negative bacteria. The extracts that were tested positive were estimated for Minimum inhibitory concentration (MIC). MIC of the extracts was determined against all bacterial strains selected for the experiment. MIC for Leaf extract was between 62.5µg/ml to 125µg/ml for Escherichia coli, 125µg/ml to 250µg/ml for Pseudomonas aeruginosa, 62.5µg/ml to 125µg/ml for Bacillus subtilis and 31.25µg/ml to 62.5µg/ml for Staphylococcus aureus. Whereas MIC for Stem Bark extract was between 500µg/ml to 1000µg/ml for Escherichia coli, 250µg/ml to 500µg/ml for Pseudomonas aeruginosa, 62.5µg/ml to 125µg/ml for Bacillus subtilis and 62.5µg/ml to 125µg/ml for Staphylococcus aureus. Ciprofloxacin used as standard antibiotic showed MIC between 1.953 µg/ml to7.813 µg/ml for all four bacterial strains. For screening of anti-fungal activity of plant Macaranga peltata two fungal strains Aspergillus niger and Candida albicans were selected. There was no antifungal activity observed by the extracts at the concentrations tested.

CONCLUSION

Macaranga peltata yield obtained from leaf was 47% and from Stem Bark was 30%. Both plant extracts were subjected to phytochemical analysis. Leaf extract showed the presence of carbohydrates, steroids and sterols, glycisides, flavonoids, tannins, proteins and amino acids while Stem Bark extract showed positive results for carbohydrates, glycosides, saponins, flavonoids, tannins, proteins and amino acids. Biological studies of plant extracts indicate antimicrobial activity. Leaf and Stem Bark samples extracts of Macaranga peltata were subjected to anti microbial studies. Both the extracts proved moderate anti bacterial activity among them leaf extracts showed better anti-bacterial activity than the Stem Bark extract against both Gram-positive and gram-negative bacteria.

ACKNOWLEDGEMENTS

We thank Manipal University for providing the facilities and support to conduct this study. Authors acknowledge the great help received from the scholars whose articles cited and included in references of this manuscript. The authors are also grateful to authors / editors / publishers of all those articles, journals and books from where the literature for this article has been reviewed and discussed. Authors are grateful to IJCRR editorial board members and IJCRR team of reviewers who have helped to bring quality to this manuscript.

Inference: Anti-bacterial activity was obtained only for one Gram-positive and one Gramnegative bacteria out of two gram-positive and two Gram-negative bacterial strains. Leaf extract showed better activity against both Gram-positive and Gram-negative bacteria. DMSO used as solvent control, did not inhibit growth of bacteria. Activity of the extracts was found to be less than the standard antibiotic Ciprofloxacin.

Inference: Very low concentration i.e. between 3.906 µg/ml to7.813 µg/ml of ciprofloxacin (used as standard anti-biotic) was able to inhibit the bacterial growth. Leaf extract inhibited the bacterial growth between 62.5µg/ml to 125µg/ml concentration while Stem Bark extract inhibited growth of bacteria between 500µg/ml to 1000µg/ml of concentration. DMSO at its higher concentrations was found to inhibit bacterial growth. Although activity showed by plant extracts was lesser as compared to standard, still Leaf extract showed better activity than Stem Bark extracts.

Very low concentration i.e. between 1.953 µg/ml to 3.906 µg/ml of ciprofloxacin (used as standard anti-biotic) was able to inhibit the bacterial growth. Leaf extract inhibited the bacterial growth between 125µg/ml to 250µg/ml concentration while Stem Bark extract inhibited growth of bacteria between 250µg/ml to 500µg/ml of concentration. DMSO at its higher concentrations was found to inhibit bacterial growth. Although activity showed by plant extracts was lesser as compared to standard, still Leaf extract showed better activity than Stem Bark extracts.

Inference: Very low concentration i.e. between 1.953 µg/ml to 3.906 µg/ml of ciprofloxacin (used as standard anti-biotic) was able to inhibit the bacterial growth. Both Leaf extract and Stem Bark extracts inhibited the bacterial growth between 62.5µg/ml to 125µg/ml concentration. DMSO at its higher concentrations was found to inhibit bacterial growth. Although activity showed by plant extracts was less, still it was comparable with standard.

Inference: Very low concentration i.e. between 1.953 µg/ml to 3.906 µg/ml of ciprofloxacin (used as standard anti-biotic) was able to inhibit the bacterial growth. Leaf extract inhibited the bacterial growth between 31.25µg/ml to 62.5µg/ml concentration while Stem Bark extract inhibited growth of bacteria between 62.5µg/ml to 125µg/ml of concentration. DMSO at its higher concentrations was found to inhibit bacterial growth. Although activity showed by plant extracts was lesser as compared to standard, still Leaf extract showed better activity than Stem Bark extracts.

References:

1. Mahesh B. and. Satish S.; Antimicrobial Activity of Some Important Medicinal Plant against Plant and Human Pathogens; World Journal of Agricultural Sciences; 2008; 4 (S): 839-843.

2. Duraipandiyan V.,Ayyanar M.and Ignacimuthu S.; Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India; BMC Complementary and Alternative Medicine 2006, 6:35

3. Lino A., Deogracious O.; The in-vitro antibacterial activity of Annona senegalensis , Securidacca longipendiculata and Steganotaenia araliacea - Ugandan medicinal plants; African Health Sciences; 2006; 6(1): 31-35

4. Uniyal S.K., Singh K.N., Jamwal P. and Lal B.; Traditional use of medicinal plants among the tribal communities of Chhota Bhangal, Western Himalaya; Journal of Ethnobiology and Ethnomedicine; 2006; 2:14

5. Yoder B. J., Cao S., Norris A., Miller J. S., Ratovoson F., Razafitsalama J., Andriantsiferana R., Rasamison V., and Kingston D; Cytotoxic Prenylated Stilbenes and Flavonoids from Macaranga alnifolia from the Madagascar Rainforest; Journal of Natural Products. 2007 March; 70(3): 342– 346

6. Srinivasan D., Sangeetha N., Suresh T and Perumalswamy PL. Antimicroabial activity of certain Indian medicinal plants used in folkloric medicine. Journal of Ethano Pharmacology. 2001; 74 (3): 217- 220.

7. Khandelwal, K. R. Practical Pharmacognosy, Techniques and Experiments, 8th Ed., Nirali Prakashan. 2001, pp. 45-69.

8. Pao-Chuan H., Jeng-Leun M., Shu-Hui H. Antimicrobial effect of various combinations of plant exracts. Food Microbiology 2001; 18 (1); 35- 43.

9. Laura LZ. Spices and Herbs: Their Antimicrobial activity and its determination. Journal of Food Safety 1988; 9 (2); 97-118.

10. Jennifer MA. Determination of minimum inhibitory concentrations. Journal of antimicrobial Chemotherapy 2001; 48 (suppl 1); 5-16.

Announcements

Dr. Pramod Kumar Manjhi joined Editor-in-Chief since July 2021 onwards

COPE guidelines for Reviewers

SCOPUS indexing: 2014, 2019 to 2021


Awards, Research and Publication incentive Schemes by IJCRR

Best Article Award: 

One article from every issue is selected for the ‘Best Article Award’. Authors of selected ‘Best Article’ are rewarded with a certificate. IJCRR Editorial Board members select one ‘Best Article’ from the published issue based on originality, novelty, social usefulness of the work. The corresponding author of selected ‘Best Article Award’ is communicated and information of award is displayed on IJCRR’s website. Drop a mail to editor@ijcrr.com for more details.

Women Researcher Award:

This award is instituted to encourage women researchers to publish her work in IJCRR. Women researcher, who intends to publish her research work in IJCRR as the first author is eligible to apply for this award. Editorial Board members decide on the selection of women researchers based on the originality, novelty, and social contribution of the research work. The corresponding author of the selected manuscript is communicated and information is displayed on IJCRR’s website. Under this award selected women, the author is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.

Emerging Researcher Award:

‘Emerging Researcher Award’ is instituted to encourage student researchers to publish their work in IJCRR. Student researchers, who intend to publish their research or review work in IJCRR as the first author are eligible to apply for this award. Editorial Board members decide on the selection of student researchers for the said award based on originality, novelty, and social applicability of the research work. Under this award selected student researcher is eligible for publication incentives. Drop a mail to editor@ijcrr.com for more details.


Best Article Award

A study by Dorothy Ebere Adimora et al. entitled \"Remediation for Effects of Domestic Violence on Psychological well-being, Depression and Suicide among Women During COVID-19 Pandemic: A Cross-cultural Study of Nigeria and Spain\" is awarded Best Article of Vol 14 issue 23
A study by Muhas C. et al. entitled \"Study on Knowledge & Awareness About Pharmacovigilance Among Pharmacists in South India\" is awarded Best article for Vol 14 issue 22
A study by Saurabh Suvidha entitled \"A Case of Mucoid Degeneration of Uterine Fibroid with Hydrosalphinx and Ovarian Cyst\" is awarded Best article of Vol 14 issue 21
A study by Alice Alice entitled \"Strengthening of Human Milk Banking across South Asian Countries: A Next Step Forward\" is awarded Best article of Vol 14 issue 20
A study by Sathyanarayanan AR et al. entitled \"The on-task Attention of Individuals with Autism Spectrum Disorder-An Eye Tracker Study Using Auticare\" is awarded Best article of Vol 14 issue 19
A study by Gupta P. et al. entitled \"A Short Review on \"A Novel Approach in Fast Dissolving Film & their Evaluation Studies\" is awarded Best Article of Vol 14 issue 18.
A study by Shafaque M. et al. entitled \"A Case-Control Study Performed in Karachi on Inflammatory Markers by Ciprofloxacin and CoAmoxicillin in Patients with Chronic Suppurative Otitis Media\" is awarded Best Article of Vol 14 issue 17
A study by Ali Nawaz et al. entitled \"A Comparative Study of Tubeless versus Standard Percutaneous Nephrolithotomy (PCNL) \? A Randomized Controlled Study\" is awarded Best Article for Vol 14 issue 16.
A study by Singh R. et al. entitled \"A Prospective Study to Find the Association of Astigmatism in Patients of Vernal Keratoconjunctivitis (VKC) in a Tertiary Health Care Centre in India (Vindhya Region MP)\" is awarded Best Article for Vol 14 issue 15
A Study by Humaira Tahir et al. entitled "Comparison of First Analgesic Demand after Major Surgeries of Obstetrics and Gynecology between Pre-Emptive Versus Intra-Operative Groups by Using Intravenous Paracetamol: A Cross-Sectional Study" is awarded Best Article for Vol 14 issue 14
A Study by Monica K. entitled "Risk Predictors for Lymphoma Development in Sjogren Syndrome - A Systematic Review" is awarded Best Article for Vol 14 issue 13
A Study by Mokhtar M Sh et al. entitled "Prevalence of Hospital Mortality of Critically Ill Elderly Patients" is awarded Best Article for Vol 14 issue 12
A Study by Vidya S. Bhat et al. entitled "Effect of an Indigenous Cleanser on the Microbial Biofilm on Acrylic Denture Base - A Pilot Study" is awarded Best Article for Vol 14 issue 11
A Study by Pandya S. et al. entitled "Acute and 28-Day Repeated Dose Subacute Toxicological Evaluation of Coroprotect Tablet in Rodents" is awarded Best Article for Vol 14 issue 10
A Study by Muhammad Zaki et al. entitled "Effect of Hemoglobin Level on the Severity of Acute Bronchiolitis in Children: A Case-Control Study" is awarded Best Article for Vol 14 issue 09
A Study by Vinita S & Ayushi S entitled "Role of Colour Doppler and Transvaginal Sonography for diagnosis of endometrial pathology in women presenting with Abnormal Uterine Bleeding" is awarded Best Article for Vol 14 issue 08
A Study by Prabhu A et al. entitled "Awareness of Common Eye Conditions among the ASHA (Accredited Social Health Activist) Workers in the Rural Communities of Udupi District- A Pilot Study" is awarded Best Article for Vol 14 issue 07
A Study by Divya MP et al. entitled "Non-Echoplanar Diffusion-Weighted Imaging and 3D Fiesta Magnetic Resonance Imaging Sequences with High Resolution Computed Tomography Temporal Bone in Assessment and Predicting the Outcome of Chronic Suppurative Otitis Media with Cholesteatoma" is awarded Best Article for Vol 14 issue 06
A Study by Zahoor Illahi Soomro et al. entitled "Functional Outcomes of Fracture Distal Radius after Fixation with Two Different Plates: A Retrospective Comparative Study" is awarded Best Article for Vol 14 issue 05
A Study by Ajai KG & Athira KN entitled "Patients’ Gratification Towards Service Delivery Among Government Hospitals with Particular Orientation Towards Primary Health Centres" is awarded Best Article for Vol 14 issue 04
A Study by Mbungu Mulaila AP et al. entitled "Ovarian Pregnancy in Kindu City, D.R. Congo - A Case Report" is awarded Best Article for Vol 14 issue 03
A Study by Maryam MJ et al. entitled "Evaluation Serum Chemerin and Visfatin Levels with Rheumatoid Arthritis: Possible Diagnostic Biomarkers" is awarded Best Article for Vol 14 issue 02
A Study by Shanthan KR et al. entitled "Comparison of Ultrasound Guided Versus Nerve Stimulator Guided Technique of Supraclavicular Brachial Plexus Block in Patients Undergoing Upper Limb Surgeries" is awarded Best Article for Vol 14 issue 01
A Study by Amol Sanap et al. entitled "The Outcome of Coxofemoral Bypass Using Cemented Bipolar Hemiarthroplasty in the Treatment of Unstable Intertrochanteric Fracture of Femur in a Rural Setup" is awarded Best Article Award of Vol 13 issue 24
A Study by Manoj KP et al. entitled "A Randomized Comparative Clinical Trial to Know the Efficacy of Ultrasound-Guided Transversus Abdominis Plane Block Against Multimodal Analgesia for Postoperative Analgesia Following Caesarean Section" is awarded Best Article Award of Vol 13 issue 23
A Study by Karimova II et al. entitled "Changes in the Activity of Intestinal Carbohydrases in Alloxan-Induced Diabetic Rats and Their Correction with Prenalon" is awarded Best Article of Vol 13 issue 22
A Study by Ashish B Roge et al. entitled "Development, Validation of RP-HPLC Method and GC MS Analysis of Desloratadine HCL and It’s Degradation Products" is awarded Best Article of Vol 13 issue 21
A Study by Isha Gaurav et al. entitled "Association of ABO Blood Group with Oral Cancer and Precancer – A Case-control Study" is awarded Best Article for Vol 13 issue 20
A Study by Amr Y. Zakaria et al. entitled "Single Nucleotide Polymorphisms of ATP-Binding Cassette Gene(ABCC3 rs4793665) affect High Dose Methotrexate-Induced Nephrotoxicity in Children with Osteosarcoma" is awarded Best Article for Vol 13 issue 19
A Study by Kholis Ernawati et al. entitled "The Utilization of Mobile-Based Information Technology in the Management of Dengue Fever in the Community Year 2019-2020: Systematic Review" is awarded Best Article for Vol 13 issue 18
A Study by Bhat Asifa et al. entitled "Efficacy of Modified Carbapenem Inactivation Method for Carbapenemase Detection and Comparative Evaluation with Polymerase Chain Reaction for the Identification of Carbapenemase Producing Klebsiella pneumonia Isolates" is awarded Best Article for Vol 13 issue 17
A Study by Gupta R. et al. entitled "A Clinical Study of Paediatric Tracheostomy: Our Experience in a Tertiary Care Hospital in North India" is awarded Best Article for Vol 13 issue 16
A Study by Chandran Anand et al. entitled "A Prospective Study on Assessment of Quality of Life of Patients Receiving Sorafenib for Hepatocellular Carcinoma" is awarded Best article for Vol 13 issue 15
A Study by Rosa PS et al. entitled "Emotional State Due to the Covid – 19 Pandemic in People Residing in a Vulnerable Area in North Lima" is awarded Best Article for Vol 13 issue 14
A Study by Suvarna Sunder J et al. entitled "Endodontic Revascularization of Necrotic Permanent Anterior Tooth with Platelet Rich Fibrin, Platelet Rich Plasma, and Blood Clot - A Comparative Study" is awarded Best Article for Vol 13 issue 13
A Study by Mona Isam Eldin Osman et al. entitled "Psychological Impact and Risk Factors of Sexual Abuse on Sudanese Children in Khartoum State" is awarded Best Article for Vol 13 issue 12
A Study by Khaw Ming Sheng & Sathiapriya Ramiah entitled "Web Based Suicide Prevention Application for Patients Suffering from Depression" is awarded Best Article for Vol 13 issue 11
A Study by Purushottam S. G. et al. entitled "Development of Fenofibrate Solid Dispersions for the Plausible Aqueous Solubility Augmentation of this BCS Class-II Drug" is awarded Best article for Vol 13 issue 10
A Study by Kumar S. et al. entitled "A Study on Clinical Spectrum, Laboratory Profile, Complications and Outcome of Pediatric Scrub Typhus Patients Admitted to an Intensive Care Unit from a Tertiary Care Hospital from Eastern India" is awarded Best Article for Vol 13 issue 09
A Study by Mardhiah Kamaruddin et al. entitled "The Pattern of Creatinine Clearance in Gestational and Chronic Hypertension Women from the Third Trimester to 12 Weeks Postpartum" is awarded Best Article for Vol 13 issue 08
A Study by Sarmila G. B. et al. entitled "Study to Compare the Efficacy of Orally Administered Melatonin and Clonidine for Attenuation of Hemodynamic Response During Laryngoscopy and Endotracheal Intubation in Gastrointestinal Surgeries" is awarded Best Article for Vol 13 issue 07
A Study by M. Muthu Uma Maheswari et al. entitled "A Study on C-reactive Protein and Liver Function Tests in Laboratory RT-PCR Positive Covid-19 Patients in a Tertiary Care Centre – A Retrospective Study" is awarded Best Article of Vol 13 issue 06 Special issue Modern approaches for diagnosis of COVID-19 and current status of awareness
A Study by Gainneos PD et al. entitled "A Comparative Evaluation of the Levels of Salivary IgA in HIV Affected Children and the Children of the General Population within the Age Group of 9 – 12 Years – A Cross-Sectional Study" is awarded Best Article of Vol 13 issue 05 Special issue on Recent Advances in Dentistry for better Oral Health
A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" is awarded Best Article of Vol 13 issue 06
A Study by Chen YY and Ghazali SRB entitled "Lifetime Trauma, posttraumatic stress disorder Symptoms and Early Adolescence Risk Factors for Poor Physical Health Outcome Among Malaysian Adolescents" is awarded Best Article of Vol 13 issue 04 Special issue on Current Updates in Plant Biology to Medicine to Healthcare Awareness in Malaysia
A Study by Kumari PM et al. entitled "Study to Evaluate the Adverse Drug Reactions in a Tertiary Care Teaching Hospital in Tamilnadu - A Cross-Sectional Study" is awarded Best Article for Vol 13 issue 05
A Study by Anu et al. entitled "Effectiveness of Cytological Scoring Systems for Evaluation of Breast Lesion Cytology with its Histopathological Correlation" is awarded Best Article of Vol 13 issue 04
A Study by Sharipov R. Kh. et al. entitled "Interaction of Correction of Lipid Peroxidation Disorders with Oxibral" is awarded Best Article of Vol 13 issue 03
A Study by Tarek Elwakil et al. entitled "Led Light Photobiomodulation Effect on Wound Healing Combined with Phenytoin in Mice Model" is awarded Best Article of Vol 13 issue 02
A Study by Mohita Ray et al. entitled "Accuracy of Intra-Operative Frozen Section Consultation of Gastrointestinal Biopsy Samples in Correlation with the Final Histopathological Diagnosis" is awarded Best Article for Vol 13 issue 01
A Study by Badritdinova MN et al. entitled "Peculiarities of a Pain in Patients with Ischemic Heart Disease in the Presence of Individual Combines of the Metabolic Syndrome" is awarded Best Article for Vol 12 issue 24
A Study by Sindhu Priya E S et al. entitled "Neuroprotective activity of Pyrazolone Derivatives Against Paraquat-induced Oxidative Stress and Locomotor Impairment in Drosophila melanogaster" is awarded Best Article for Vol 12 issue 23
A Study by Habiba Suhail et al. entitled "Effect of Majoon Murmakki in Dysmenorrhoea (Usre Tams): A Standard Controlled Clinical Study" is awarded Best Article for Vol 12 issue 22
A Study by Ghaffar UB et al. entitled "Correlation between Height and Foot Length in Saudi Population in Majmaah, Saudi Arabia" is awarded Best Article for Vol 12 issue 21
A Study by Siti Sarah Binti Maidin entitled "Sleep Well: Mobile Application to Address Sleeping Problems" is awarded Best Article for Vol 12 issue 20
A Study by Avijit Singh"Comparison of Post Operative Clinical Outcomes Between “Made in India” TTK Chitra Mechanical Heart Valve Versus St Jude Mechanical Heart Valve in Valve Replacement Surgery" is awarded Best Article for Vol 12 issue 19
A Study by Sonali Banerjee and Mary Mathews N. entitled "Exploring Quality of Life and Perceived Experiences Among Couples Undergoing Fertility Treatment in Western India: A Mixed Methodology" is awarded Best Article for Vol 12 issue 18
A Study by Jabbar Desai et al. entitled "Prevalence of Obstructive Airway Disease in Patients with Ischemic Heart Disease and Hypertension" is awarded Best Article for Vol 12 issue 17
A Study by Juna Byun et al. entitled "Study on Difference in Coronavirus-19 Related Anxiety between Face-to-face and Non-face-to-face Classes among University Students in South Korea" is awarded Best Article for Vol 12 issue 16
A Study by Sudha Ramachandra & Vinay Chavan entitled "Enhanced-Hybrid-Age Layered Population Structure (E-Hybrid-ALPS): A Genetic Algorithm with Adaptive Crossover for Molecular Docking Studies of Drug Discovery Process" is awarded Best article for Vol 12 issue 15
A Study by Varsha M. Shindhe et al. entitled "A Study on Effect of Smokeless Tobacco on Pulmonary Function Tests in Class IV Workers of USM-KLE (Universiti Sains Malaysia-Karnataka Lingayat Education Society) International Medical Programme, Belagavi" is awarded Best article of Vol 12 issue 14, July 2020
A study by Amruta Choudhary et al. entitled "Family Planning Knowledge, Attitude and Practice Among Women of Reproductive Age from Rural Area of Central India" is awarded Best Article for special issue "Modern Therapeutics Applications"
A study by Raunak Das entitled "Study of Cardiovascular Dysfunctions in Interstitial Lung Diseas epatients by Correlating the Levels of Serum NT PRO BNP and Microalbuminuria (Biomarkers of Cardiovascular Dysfunction) with Echocardiographic, Bronchoscopic and HighResolution Computed Tomography Findings of These ILD Patients" is awarded Best Article of Vol 12 issue 13 
A Study by Kannamani Ramasamy et al. entitled "COVID-19 Situation at Chennai City – Forecasting for the Better Pandemic Management" is awarded best article for  Vol 12 issue 12
A Study by Muhammet Lutfi SELCUK and Fatma entitled "Distinction of Gray and White Matter for Some Histological Staining Methods in New Zealand Rabbit's Brain" is awarded best article for  Vol 12 issue 11
A Study by Anamul Haq et al. entitled "Etiology of Abnormal Uterine Bleeding in Adolescents – Emphasis Upon Polycystic Ovarian Syndrome" is awarded best article for  Vol 12 issue 10
A Study by entitled "Estimation of Reference Interval of Serum Progesterone During Three Trimesters of Normal Pregnancy in a Tertiary Care Hospital of Kolkata" is awarded best article for  Vol 12 issue 09
A Study by Ilona Gracie De Souza & Pavan Kumar G. entitled "Effect of Releasing Myofascial Chain in Patients with Patellofemoral Pain Syndrome - A Randomized Clinical Trial" is awarded best article for  Vol 12 issue 08
A Study by Virendra Atam et. al. entitled "Clinical Profile and Short - Term Mortality Predictors in Acute Stroke with Emphasis on Stress Hyperglycemia and THRIVE Score : An Observational Study" is awarded best article for  Vol 12 issue 07
A Study by K. Krupashree et. al. entitled "Protective Effects of Picrorhizakurroa Against Fumonisin B1 Induced Hepatotoxicity in Mice" is awarded best article for issue Vol 10 issue 20
A study by Mithun K.P. et al "Larvicidal Activity of Crude Solanum Nigrum Leaf and Berries Extract Against Dengue Vector-Aedesaegypti" is awarded Best Article for Vol 10 issue 14 of IJCRR
A study by Asha Menon "Women in Child Care and Early Education: Truly Nontraditional Work" is awarded Best Article for Vol 10 issue 13
A study by Deep J. M. "Prevalence of Molar-Incisor Hypomineralization in 7-13 Years Old Children of Biratnagar, Nepal: A Cross Sectional Study" is awarded Best Article for Vol 10 issue 11 of IJCRR
A review by Chitra et al to analyse relation between Obesity and Type 2 diabetes is awarded 'Best Article' for Vol 10 issue 10 by IJCRR. 
A study by Karanpreet et al "Pregnancy Induced Hypertension: A Study on Its Multisystem Involvement" is given Best Paper Award for Vol 10 issue 09

List of Awardees

A Study by Ese Anibor et al. "Evaluation of Temporomandibular Joint Disorders Among Delta State University Students in Abraka, Nigeria" from Vol 13 issue 16 received Emerging Researcher Award


A Study by Alkhansa Mahmoud et al. entitled "mRNA Expression of Somatostatin Receptors (1-5) in MCF7 and MDA-MB231 Breast Cancer Cells" from Vol 13 issue 06 received Emerging Researcher Award


RSS feed

Indexed and Abstracted in


Antiplagiarism Policy: IJCRR strongly condemn and discourage practice of plagiarism. All received manuscripts have to pass through "Plagiarism Detection Software" test before Toto Macau forwarding for peer review. We consider "Plagiarism is a crime"

IJCRR Code of Conduct: To achieve a high standard of publication, we adopt Good Publishing Practices (updated in 2022) which are inspired by guidelines provided by Committee on Publication Ethics (COPE), Open Access Scholarly Publishers Association (OASPA) and International Committee of Medical Journal Editors (ICMJE)

Disclaimer: International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal.



ABOUT US

International Journal of Current Research and Review (IJCRR) provides platform for researchers to publish and discuss their original research and review work. IJCRR can not be held responsible for views, opinions and written statements of researchers published in this journal

Contact

148, IMSR Building, Ayurvedic Layout,
        Near NIT Complex, Sakkardara,
        Nagpur-24, Maharashtra State, India

editor@ijcrr.com

editor.ijcrr@gmail.com


Copyright © 2024 IJCRR. Specialized online journals by ubijournal .Website by Ubitech solutions